Journal: Neurobiology of Stress

Article Title: Microglial SIRT6 confers protection against neuroinflammation-associated depression through NRF2-HO1 signaling

doi: 10.1016/j.ynstr.2026.100804

Figure Lengend Snippet: Sirt6 regulated the microglial activation and the depression behavior through Nrf2-HO1 signaling in vivo (A) Experimental timeline of tamoxifen administration, AAV9-Cx3cr1-Nrf2 injection, LPS challenge, and behavioral tests in Sirt6 MCKO mice. (B) Western blot analysis of NRF2 levels after AAV9-Cx3cr1-Nrf2 injection. n = 3 mice. (C-D) Immobility time in TST (C) and FST (D). n = 8 mice. (E) TAC, MDA, SOD, and GSH/GSSG levels at 5 days after LPS injection. n = 4 mice. (F-L) Immunostaining of GFAP and IBA1 in the hippocampus area at 5 days after LPS injection (F); Quantification of (G) IBA1 + and (H) GFAP + cell counts, along with microglial morphology parameters: (I) number of branches, (J) average branch length, (K) total branch length, and (L) soma area. n = 4 mice. (M) qPCR analysis of TNF-α,IL-6, and IL-1β mRNA levels in the microglial sorted from the hippocampus brain tissue. n = 4 mice. (N) Western blot analysis of NRF2 downstream signaling component protein levels in microglia sorted from the hippocampus. n = 4 mice. Data are mean ± SEM. Statistical significance between two groups was determined by an unpaired two-tailed Student's t-test.

Article Snippet: Sirt6 flox/flox ( Sirt6 fl/fl )mice (Stock NM-CKO-200241) were provided by Shanghai Model Organisms, Nrf2 flox/flox ( Nrf2 fl/fl ) mice (Stock 025433) and Cx3cr1 -CreERT2 mice (Stock 021160) were obtained from the Jackson Laboratory.

Techniques: Activation Assay, In Vivo, Injection, Western Blot, Immunostaining, Two Tailed Test

Journal: Neurobiology of Stress

Article Title: Microglial SIRT6 confers protection against neuroinflammation-associated depression through NRF2-HO1 signaling

doi: 10.1016/j.ynstr.2026.100804

Figure Lengend Snippet: Overexpression of Sirt6 in microglial improved the depression behavior and impeded the microglial activation (A) Experimental timeline depicting tamoxifen administration, followed by tail vein injection of AAV9-Cx3cr1-Sirt6, subsequent LPS challenge, and finally, a series of behavioral tests. (B) Western blot analysis of SIRT6 expression after AAV9-Cx3cr1-Sirt6 injection. n = 3 mice. (C-D) The immobility time was quantified in TST (C) and FST (D). n = 8 mice. (E) TAC, MDA, SOD, and GSH/GSSG levels at 5 days after LPS injection. (F-L) Immunostaining of GFAP and IBA1 in the hippocampus area at 5 days after LPS injection (F); Quantification of (G) IBA1 + and (H) GFAP + cell counts, along with microglial morphology parameters: (I) number of branches, (J) average branch length, (K) total branch length, and (L) soma area. n = 4 mice.(M) qPCR analysis of TNF-α,IL-6, and IL-1β mRNA levels in the microglial sorted from the hippocampus brain tissue. n = 4 mice. Data are mean ± SEM. Statistical significance between two groups was determined by an unpaired two-tailed Student's t-test.

Article Snippet: Sirt6 flox/flox ( Sirt6 fl/fl )mice (Stock NM-CKO-200241) were provided by Shanghai Model Organisms, Nrf2 flox/flox ( Nrf2 fl/fl ) mice (Stock 025433) and Cx3cr1 -CreERT2 mice (Stock 021160) were obtained from the Jackson Laboratory.

Techniques: Over Expression, Activation Assay, Injection, Western Blot, Expressing, Immunostaining, Two Tailed Test

Journal: Nature Communications

Article Title: Pericytes are organ-specific regulators of tissue morphogenesis

doi: 10.1038/s41467-026-71643-1

Figure Lengend Snippet: a Labeling of pericytes in the P12 brain cortex via Pdgfrb(BAC)-CreERT2 -mediated, tamoxifen-induced GFP expression (green). Confocal images also show PDGFR (gray), a pericyte marker, and ICAM2, labeling ECs (red). Bottom panels show higher magnification of the yellow dashed boxes in the top row. b Confocal images showing the proximity of GFP+ pericytes (green), Glial fibrillary acidic protein (GFAP)+ astrocytes (magenta) and Allograft inflammatory factor-1 (AIF)+ microglia (gray) in the P12 brain cortex. c Pdgfrb(BAC)-CreERT2 -mediated labeling of pericytes (GFP, green) in the P21 lung. Confocal images also show PDGFRβ (gray) and ICAM2 (red). d Maximum intensity projections showing PDGFRβ+ pericytes (gray) and ICAM2+ ECs (red) in the lung alveolus. Red arrows indicate pulmonary pericytes in the alveolar septum. e Confocal images showing the localization of Prosurfactant Protein C (proSP-C)-expressing type 2 alveolar epithelial cells (green) and receptor for advanced glycation end products (RAGE)-positive type 1 alveolar epithelial cells (gray). f Graphs showing the number (per area/82 × 82 × 6 µm) of pericytes and type 2 alveolar epithelial cells in the septal and alveolar regions of lung alveoli. Data represent mean ± s.e.m. ( n = 5 mice per group); P -values, unpaired two-tailed Student t -test (pericytes) and two-tailed Mann–Whitney test (alveolar epithelial cells). Volcano plots showing the differential expression of genes ( g ) and differentially expressed ligands ( h ) in pericytes from P14 lung versus P12 brain. Average log2-fold change > 0.6, adjusted p -value < 0.05). i Heatmap of differentially expressed ligands (padj < 0.01) in pericytes from postnatal lung versus brain. Pseudobulk DE analysis uses two-sided Wald test + independent filtering as implemented by pyDESeq2 ( g – i ). Source data are provided as a Source Data file.

Article Snippet: In vivo labeling of pericytes was performed by mating Pdgfrb(BAC)-CreERT2 transgenic animals (available as strain #029684 from The Jackson Laboratory) and Rosa26 mT/mG reporter mice .

Techniques: Labeling, Expressing, Marker, Two Tailed Test, MANN-WHITNEY, Quantitative Proteomics

Journal: bioRxiv

Article Title: Neuromodulation of Foxp2 + hypothalamic neurons induces therapeutic hypothermia

doi: 10.64898/2026.05.04.722579

Figure Lengend Snippet: a , Average relative cerebral blood volume changes (ΔrCBV) of different region across the brain within 5 to 10 minutes after administration P57 compared to vehicle. b, 3D rendering of ΔrCBV of different hypothalamic nuclei within 5 to 10 minutes after administration P57 compared to vehicle. c, Different views of the 3D rendering results of various P57-activated brain regions in the hypothalamus within 5 to 10 minutes after administration. d, Schematic of labeling P57 activated neurons using Fos-CreERT2;tdTomato mice (n=6 mice for each group). e, f, Number of tdTomato + neurons across different hypothalamic nuclei (e) and representative slice (top) and quantification (bottom) of MPA and PVH (f) in P57 group and Vehicle group in Fos-CreERT2;tdTomato mice. Student’s t test used was two-sided. Heat-map values are means; floating bars show min to max and mean (line). Bar chart and error bars are presented as mean values ± s.e.m. Median eminence (ME), Paraventricular hypothalamic nucleus (PVH), Hypothalamic medial zone (MEZ),

Article Snippet: For whole brain imaging and virus injection, we used adult (6-10-week-old) male and female Fos-CreERT2;tdTomato mice (Jackson Lab), Fos-CreERT2 mice (Jackson Lab), and Foxp2-Cre mice (Jackson Lab).

Techniques: Labeling

Journal: bioRxiv

Article Title: Neuromodulation of Foxp2 + hypothalamic neurons induces therapeutic hypothermia

doi: 10.64898/2026.05.04.722579

Figure Lengend Snippet: Representative slice of posterior hypothalamic nucleus (PH), dorsomedial nucleus (DMH) and mammillary body (MM) in P57 group and Vehicle group in Fos-CreERT2;tdTomato mice.

Article Snippet: For whole brain imaging and virus injection, we used adult (6-10-week-old) male and female Fos-CreERT2;tdTomato mice (Jackson Lab), Fos-CreERT2 mice (Jackson Lab), and Foxp2-Cre mice (Jackson Lab).

Techniques: